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Resolution of the stereoisomers of leucovorin and 5-methyltetrahydrofolate by chiral HPLC
Summary
Type:
HPLC
Application #:
105860
Catalog#:
720046.40 - MN HPLC Column, Resolvosil BSA-7, 300A, 7um, 4x150mm
Detection:
UV, 282nm
Conditions:
eluent: 5mM Na phosphate buffer pH 7.4
flow rate 1.0ml/min
Authors:
Choi, K.E. et al.
Source:
Anal. Biochem. 168 (1988) 398–404
Details
Legend: Fig. 2. Chromatogram of the separation of the two stereoisomers of commercially available leucovorin, 10µl of a 1mg/ml solution of [6R,S]-LV. Biologically active [6S]-isomer: first eluted peak (RT =
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